Sickle confirm modified hemoglobin solubility testTitle:
Sickle confirm modified hemoglobin solubility test
Inventors: Tim R. Randolph
The present invention provides a method for determining sickle-cell zygosity in a subject, comprising: forming a first solution comprising a sample from the subject, a phosphate buffer, a detergent, and a reducing agent; subjecting the first solution to centrifugation to form a second solution and a supernatant; taking a color reading of the supernatant and of the second solution; optionally filtering the second solution to form a filtrate and a precipitate, and optionally measuring the amount of the precipitation and the absorbance of the filtrate or taking a color reading of the filtrate.
U.S. patent pending
Stephanie Kimzey, MBA