16-006 - Integration of three-dimensional cell culture scaffolds in microfluidic devices by direct electrospinning

Researchers

Robert S. Martin, Chengpen Chen and Scott A. Sell

Summary

Saint Louis University researchers have developed a novel extracellular-matrix-like fibrous substrate for encapsulating living cells into a flow chamber for potential cell studies under dynamic flow conditions using a novel method of direct electrospinning fibers into a fluidic device. The method eliminates the overspray and deposition problems of electrospun fibers utilizing traditional techniques and dynamically focused fibers into a fluidic channel.  Fine fibers and porous structures can be constructed on the inside of the channel as a potential cell culture matrix. Results indicate cell adhesion and proliferation in their physiologic morphology. This work provides a new protocol for cell culture in a fluidic device, which can potentially be applied to the development of novel physiological models for scientific research and drug development.

Intellectual Property Status

• U.S. patent pending